Formation of superoxide anion and carbon-centered radicals by photosystem II under high light and heat stress-EPR spin-trapping study

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33145242" target="_blank" >RIV/61989592:15310/13:33145242 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10863-013-9523-y" target="_blank" >http://dx.doi.org/10.1007/s10863-013-9523-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10863-013-9523-y" target="_blank" >10.1007/s10863-013-9523-y</a>

Result language
angličtina
Original language name
Formation of superoxide anion and carbon-centered radicals by photosystem II under high light and heat stress-EPR spin-trapping study
Original language description
In this study, electron paramagnetic resonance spin-trapping technique was used to study the light-induced production of superoxide anion (O2?-) and carbon-centered (R?) radicals by Photosystem II (PSII). It is evidenced here that exposure of PSII membranes to high light (2000 micromol photons m-2 s-1) or heat (47 °C) treatments prior to the illumination suppressed O2?- production, while R? was formed. Formation of R? in the both high light- and heat-treated PSII membranes was enhanced by DCMU. Removalof molecular oxygen by glucose/glucose oxidase/catalase system and O2?- scavenging by exogenous superoxide dismutase completely suppressed carbon-centered radical formation. It is proposed here that the oxidation of polyunsaturated fatty or amino acid byO2?- on the electron acceptor side of PSII results in the formation of R?, known to initiate a cascade reaction leading to the lipid peroxidation and protein degradation.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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