Vesicular Trafficking and Stress Response Coupled to PI3K Inhibition by LY294002 as Revealed by Proteomic and Cell Biological Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33146711" target="_blank" >RIV/61989592:15310/13:33146711 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/pr400466x" target="_blank" >http://dx.doi.org/10.1021/pr400466x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/pr400466x" target="_blank" >10.1021/pr400466x</a>

Result language
angličtina
Original language name
Vesicular Trafficking and Stress Response Coupled to PI3K Inhibition by LY294002 as Revealed by Proteomic and Cell Biological Analysis
Original language description
LY294002 is a synthetic quercetin-like compound, which, unlike wortmannin, is more specific inhibitor of phosphatidylinositol 3-kinase (PI3K). It inhibits endocytosis and vacuolar transport. We report here on the proteome-wide effects of LY294002 on Arabidopsis roots focusing on proteins involved in vesicular trafficking and stress response. At the subcellular level, LY294002 caused swelling and clustering of late endosomes leading to inhibition of vacuolar transport. At the proteome level, this compound caused changes in abundances of proteins categorized to 10 functional classes. Among proteins involved in vesicular trafficking, a small GTPase ARFA1f was more abundant, indicating its possible contribution to the aggregation and fusion of late endosomes triggered by LY294002. Our study provides new information on storage proteins and vacuolar hydrolases in vegetative tissues treated by LY294002. Vacuolar hydrolases were downregulated, while storage proteins were more abundant, suggest
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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