In vitro characterisation of the anti-intravasative properties of the marine product heteronemin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33148029" target="_blank" >RIV/61989592:15310/13:33148029 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs00204-013-1045-1" target="_blank" >http://link.springer.com/article/10.1007%2Fs00204-013-1045-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00204-013-1045-1" target="_blank" >10.1007/s00204-013-1045-1</a>

Result language

angličtina

Original language name

In vitro characterisation of the anti-intravasative properties of the marine product heteronemin

Original language description

Metastases destroy the function of infested organs and are the main reason of cancer-related mortality. Heteronemin, a natural product derived from a marine sponge, was tested in vitro regarding its properties to prevent tumour cell intravasation throughthe lymph-endothelial barrier. In three-dimensional (3D) cell cultures consisting of MCF-7 breast cancer cell spheroids that were placed on lymph-endothelial cell (LEC) monolayers, tumour cell spheroids induce "circular chemorepellent-induced defects" (CCIDs) in the LEC monolayer; 12(S)-Hydroxyeicosatetraenoic acid (12(S)-HETE) and NF-kappa B activity are major factors inducing CCIDs, which are entry gates for tumour emboli intravasating the vasculature. This 3D co-culture is a validated model for theinvestigation of intravasation mechanisms and of drugs preventing CCID formation and hence lymph node metastasis. Furthermore, Western blot analyses, NF-kappa B reporter, EROD, SELE, 12(S)-HETE, and adhesion assays were performed to inves

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centre of the Region Haná for Biotechnological and Agricultural Research</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Toxicology

ISSN

0340-5761

e-ISSN

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Volume of the periodical

87

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

1851-1861

UT code for WoS article

000324773300008

EID of the result in the Scopus database

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