Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33148910" target="_blank" >RIV/61989592:15310/14:33148910 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/14:33148910
Result on the web
<a href="http://dx.doi.org/10.1016/j.electacta.2013.06.115" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2013.06.115</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2013.06.115" target="_blank" >10.1016/j.electacta.2013.06.115</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes
Original language description
This study focuses on application of room temperature ionic liquids (RTILs) as solubilizers, adsorption solvents and supporting electrolytes for electrochemical analysis of human and bovine serum albumins, HSA and BSA. The proteins were analyzed by ex situ, adsorptive transfer, square-wave voltammetry (SWV) at a basal-plane pyrolytic graphite electrode after solubilization using imidazolium- and ammonium-based RTILs. The application of RTILs enabled SWV scan from 0 to +1.5 V (vs. Ag/AgCl/3 M KCl) without interference with the anodic response of the proteins. Concretely, Tyr (Y) and Trp (W) oxidation currentsof HSA and BSA, peak Y&W around +0.85 V, were observed and characterized under different RTILs and RTIL/water conditions. The electrochemical datawere supported by electrophoresis under denaturing and native conditions. These provided evidence for the structural changes and stability of the studied proteinsin the presence of RTILs. The data acquired using BSA and HSA model proteins
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0057" target="_blank" >EE2.3.20.0057: Progress and internationalization of biophysical research at Faculty of Science, Palacky University in Olomouc</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica Acta
ISSN
0013-4686
e-ISSN
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Volume of the periodical
126
Issue of the periodical within the volume
April
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
31-36
UT code for WoS article
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EID of the result in the Scopus database
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