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Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33152646" target="_blank" >RIV/61989592:15310/14:33152646 - isvavai.cz</a>

  • Alternative codes found

    RIV/60077344:_____/14:00439433 RIV/00159816:_____/14:00061216 RIV/60076658:12520/14:43886922

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S0968432814001565" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0968432814001565</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.micron.2014.08.001" target="_blank" >10.1016/j.micron.2014.08.001</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

  • Original language description

    When developing new nanoparticles for bio-applications, it is important to fully characterize the nanoparticle's behavior in biological systems. The most common techniques employed for mapping nanoparticles inside cells include transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). These techniques entail passing an electron beam through a thin specimen. STEM or TEM imaging is often used for the detection of nanoparticles inside cellular organelles. However, lengthysample preparation is required (i.e., fixation, dehydration, drying, resin embedding, and cutting). In the present work, a new matrix (FTO glass) for biological samples was used and characterized by field emission scanning electron microscopy (FE-SEM) to generate images comparable to those obtained by TEM. Using FE-SEM, nanoparticle images were acquired inside endo/lysosomes without disruption of the cellular shape. Furthermore, the initial steps of nanoparticle incorporation into the c

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Micron

  • ISSN

    0968-4328

  • e-ISSN

  • Volume of the periodical

    67

  • Issue of the periodical within the volume

    DEC

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

    149-154

  • UT code for WoS article

    000343641300019

  • EID of the result in the Scopus database