Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33153246" target="_blank" >RIV/61989592:15310/14:33153246 - isvavai.cz</a>
Result on the web
<a href="http://download.springer.com/static/pdf/368/art%253A10.1007%252Fs00284-014-0659-8.pdf?auth66=1425291013_36c3ff24ed4b99c1445b6a6e3b172c26&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/368/art%253A10.1007%252Fs00284-014-0659-8.pdf?auth66=1425291013_36c3ff24ed4b99c1445b6a6e3b172c26&ext=.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00284-014-0659-8" target="_blank" >10.1007/s00284-014-0659-8</a>
Alternative languages
Result language
angličtina
Original language name
Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic
Original language description
The variation in the diversity of methanogens in sediment depths from Sitka stream was studied by constructing a 16S rRNA gene library using methanogen-specific primers and a denaturing gradient gel electrophoresis (DGGE)-based approach. A total of ninedifferent phylotypes from the 16S rRNA library were obtained, and all of them were clustered within the order Methanosarcinales. These nine phylotypes likely represent nine new species and at least 5-6 new genera. Similarly, DGGE analysis revealed an increase in the diversity of methanogens with an increase in sediment depth. These results suggest that Methanosarcinales phylotypes might be the dominant methanogens in the sediment from Sitka stream, and the diversity of methanogens increases as the depthincreases. Results of the present study will help in making effective strategies to monitor the dominant methanogen phylotypes and methane emissions in the environment.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.30.0041" target="_blank" >EE2.3.30.0041: POST-UP II.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Microbiology
ISSN
0343-8651
e-ISSN
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Volume of the periodical
69
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
809-816
UT code for WoS article
000343913800006
EID of the result in the Scopus database
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