Methanogenic archaea diversity in hyporheic sediments of a small lowland stream

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33157828" target="_blank" >RIV/61989592:15310/15:33157828 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S1075996414001681" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1075996414001681</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anaerobe.2014.11.009" target="_blank" >10.1016/j.anaerobe.2014.11.009</a>

Result language

angličtina

Original language name

Methanogenic archaea diversity in hyporheic sediments of a small lowland stream

Original language description

Abundance and diversity of methanogenic archaea were studied at five localities along a longitudinal profile of a Sitka stream (Czech Republic). Samples of hyporheic sediments were collected from two sediment depths (0-25 cm and 25-50 cm) by freeze-coremethod. Methanogen community was analyzed by fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and sequencing method. The proportion of methanogens to the DAPI-stained cells varied among all localities and depths with an average value 2.08 x 10(5) per g of dry sediment with the range from 0.37 to 4.96 x 10(5) cells per g of dry sediment. A total of 73 bands were detected at 19 different positions on the DGGE gel and the highest methanogen diversity was found at the downstream located sites. There was no relationship between methanogen diversity and sediment depth. Cluster analysis of DGGE image showed three main clusters consisting of localities that differed in the number and similarity of the DG

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

<a href="/en/project/GA526%2F09%2F1639" target="_blank" >GA526/09/1639: Linking biogeochemisty of methane and molecular detection of methanogens and methanotrophs in river sediments.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Anaerobe

ISSN

1075-9964

e-ISSN

—

Volume of the periodical

32

Issue of the periodical within the volume

APR

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

24-31

UT code for WoS article

000353427200005

EID of the result in the Scopus database

—