Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33153560" target="_blank" >RIV/61989592:15310/14:33153560 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/14:00440405 RIV/61389030:_____/14:00440405 RIV/62156489:43210/14:00216922
Result on the web
<a href="http://download.springer.com/static/pdf/897/art%253A10.1007%252Fs00709-014-0644-x.pdf?auth66=1425563607_400ef2156c697438d32da5554b1833db&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/897/art%253A10.1007%252Fs00709-014-0644-x.pdf?auth66=1425563607_400ef2156c697438d32da5554b1833db&ext=.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00709-014-0644-x" target="_blank" >10.1007/s00709-014-0644-x</a>

Result language
angličtina
Original language name
Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes
Original language description
Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse theMT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selectedecotypes under different copper treatments to show the tissue-specific expression response to copper stress. We demonstrated that higher copper concentrations specifically affected MT3 expression among ecotypes. Our analysis shows that MT
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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