Superresolution live imaging of plant cells using structured illumination microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156443" target="_blank" >RIV/61989592:15310/15:33156443 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/15:33156443

Result on the web

<a href="http://www.nature.com/nprot/journal/v10/n8/full/nprot.2015.083.html" target="_blank" >http://www.nature.com/nprot/journal/v10/n8/full/nprot.2015.083.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nprot.2015.083" target="_blank" >10.1038/nprot.2015.083</a>

Result language

angličtina

Original language name

Superresolution live imaging of plant cells using structured illumination microscopy

Original language description

Although superresolution (SR) approaches have been routinely used for fixed or living material from other organisms, the use of time-lapse structured illumination microscopy (SIM) imaging in plant cells still remains under-developed. Here we describe a validated method for time-lapse SIM that focuses on cortical microtubules of different plant cell types. By using one of the existing commercially available SIM platforms, we provide a user-friendly and easy-to-follow protocol that may be widely applied to the imaging of plant cells. This protocol includes steps describing calibration of the microscope and channel alignment, generation of an experimental point spread function (PSF), preparation of appropriate observation chambers with available plant material, image acquisition, reconstruction and validation. This protocol can be carried out within two to three working days.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Protocols

ISSN

1754-2189

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

1248-1263

UT code for WoS article

000358482200009

EID of the result in the Scopus database

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