Evaluation of pseudotrypsin cleavage specificity towards proteins by MALDI-TOF mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156813" target="_blank" >RIV/61989592:15310/15:33156813 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/15:43875471
Result on the web
<a href="http://dx.doi.org/10.2174/0929866522666151008151617" target="_blank" >http://dx.doi.org/10.2174/0929866522666151008151617</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2174/0929866522666151008151617" target="_blank" >10.2174/0929866522666151008151617</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of pseudotrypsin cleavage specificity towards proteins by MALDI-TOF mass spectrometry
Original language description
Trypsin is a protease, which is commonly used for the digestion of protein samples in proteomic experiments. The process of trypsin autolysis is known to produce autolytic peptides as well as active enzyme forms with one or more intra-chain splits. In consequence, their variable presence can influence the digestion of a protein substrate in the reaction mixture. Besides two major and well-studied forms named beta-trypsin and alpha-trypsin, there are also other active trypsin forms known such as gamma-trypsin and pseudotrypsin (psi-trypsin). In this work, the cleavage specificity of psi-trypsin was evaluated using in-gel digestion of protein standards followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and tandem mass spectrometry (MS/MS) analyses of the resulting peptides. The numbers of produced and matching peptides were similar to those obtained using alpha-/beta-trypsin. The same experience was obtained with a real complex prote
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein and Peptide Letters
ISSN
0929-8665
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
12
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
1123-1132
UT code for WoS article
000365041200011
EID of the result in the Scopus database
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