Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33160735" target="_blank" >RIV/61989592:15310/16:33160735 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs11240-016-1133-z" target="_blank" >http://link.springer.com/article/10.1007%2Fs11240-016-1133-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-016-1133-z" target="_blank" >10.1007/s11240-016-1133-z</a>

Result language

angličtina

Original language name

Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential

Original language description

Beautiful vavilovia, the closest species to the common now extinct ancestor of the whole tribe Fabeae holds signiicant taxonomical interest and also for breeding within this group of species, which includes the most cultivated leguminous pulses in the world. In spite of this, vavilovia has attracted very scarce research to date and is in danger of complete extinction. Thus, as a part of the research carried out by an informal international group of researchers from various countries, we report here various experiments for the development and exploitation of a range of biotechnology tools for vavilovia, ranging from standard in vitro propagation, to plant regeneration from explant-derived callus, and also from protoplasts. Plants were successfully recovered following propagation from nodes, and by regeneration through organogenesis from callus derived from internodes (which provided the best responses) and leaves. Also, protoplasts were isolated from leaves and stems from in vitro shoots and from callus derived from these two explants, with the latter undergoing sustained division. Subsequently, protoplasts isolated from internode callus proliferated and also underwent organogenesis coupled with whole plant recovery at a low frequency, while protoplasts from leaf callus origin followed both organogenesis and embryogenesis simultaneously but failed to yield viable plants. Flow cytometry assessments permitted to ascertain the genetic idelity of both propagated and regenerated plants irrespectively of the source tissue from which they were derived (i.e., either callus from explants or from protoplasts). Finally, low cytometry also permitted us to provide the irst record on the relative nuclear DNA content and genome size for Vavilovia formosa.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell, Tissue and Organ Culture (print)

ISSN

0167-6857

e-ISSN

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Volume of the periodical

127

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

"637-648"

UT code for WoS article

000391389700011

EID of the result in the Scopus database

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