Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33160999" target="_blank" >RIV/61989592:15310/16:33160999 - isvavai.cz</a>

Result on the web

<a href="http://journal.frontiersin.org/article/10.3389/fpls.2015.01187/full" target="_blank" >http://journal.frontiersin.org/article/10.3389/fpls.2015.01187/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2015.01187" target="_blank" >10.3389/fpls.2015.01187</a>

Result language

angličtina

Original language name

Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy

Original language description

The development of the root apex is determined by progress of cells from the meristematic region to the successive post mitotic developmental zones for transition, cell elongation and final cell differentiation. We addressed root development, tissue architecture and root developmental zonation by means of light-sheet microscopic imaging of Arabidopsis thaliana seedlings expressing END BINDING protein 1c (EB1c) fused to green fluorescent protein (GEE) under control of native EB1c promoter. Unlike the other two members of the EB1 family, plant-specific EB1c shows prominent nuclear localization in non-dividing cells in all developmental zones of the root apex. The nuclear localization of EB1c was previously mentioned solely in meristematic cells, but not further addressed. With the help of advanced light sheet microscopy, we report quantitative evaluations of developmentally-regulated nuclear levels of the EB1c protein tagged with GEE relatively to the nuclear size in diverse root tissues (epidermis, cortex, and endodermis) and root developmental zones (meristem, transition, and elongation zones). Our results demonstrate a high potential of light sheet microscopy for 4D live imaging of fluorescently-labeled nuclei in complex samples such as developing roots, showing capacity to quantify parameters at deeper cell layers (eg., endodermis) with minimal aberrations. The data presented herein further signify the unique role of developmental cell reprogramming in the transition from cell proliferation to cell differentiation in developing root apex.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/GP14-27598P" target="_blank" >GP14-27598P: Mitogen-activated protein kinase-dependent phosphorylation and functional regulation of cytoskeletal End binding 1c protein</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

JAN

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

"1187-1"-"1187-15"

UT code for WoS article

000367653100001

EID of the result in the Scopus database

2-s2.0-84956668190