Quantification of structurally related aliphatic amino alcohols in L-valinol by hydrophilic interaction liquid chromatography separation combined with postcolumn derivatization and fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161325" target="_blank" >RIV/61989592:15310/16:33161325 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201501302/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201501302/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201501302" target="_blank" >10.1002/jssc.201501302</a>

Result language

angličtina

Original language name

Quantification of structurally related aliphatic amino alcohols in L-valinol by hydrophilic interaction liquid chromatography separation combined with postcolumn derivatization and fluorescence detection

Original language description

The amino alcohols in L-valinol were effectively separated and quantified using hydrophilic interaction chromatography with fluorescence detection. The influence of the mobile phase (salt type, buffer concentration, and pH) on retention was studied. A column TSKgel amide and mobile phase consisting of 10 mM acetate buffer pH 4.0 and acetonitrile (20: 80, v/v) provided well-separated symmetric peaks of analytes. Fluorescence detection was performed using postcolumn derivatization with o-phtaldialdehyde/2-mercaptoethanol at an excitation and emission wavelength of 345 and 450 nm, respectively. Simple sample pretreatment and very high sensitivity represent the main advantages of the developed method. After validation, the method was successfully applied to the analysis of commercial samples of L-valinol.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

<a href="/en/project/LO1305" target="_blank" >LO1305: Development of the center of advanced technologies and materials</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

39

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

851-856

UT code for WoS article

000372280600006

EID of the result in the Scopus database

—