Quantitative auxin metabolite profiling using stable isotope dilution UHPLC-MS/MS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33162306" target="_blank" >RIV/61989592:15310/16:33162306 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/cppb.20028" target="_blank" >http://dx.doi.org/10.1002/cppb.20028</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cppb.20028" target="_blank" >10.1002/cppb.20028</a>

Result language
angličtina
Original language name
Quantitative auxin metabolite profiling using stable isotope dilution UHPLC-MS/MS
Original language description
Auxin, a plant hormone, is able to stimulate growth and to control developmental processes. To get a deeper level of understanding of how auxin activity is regulated in planta, we have developed a sensitive and selective mass spectrometry-based protocol for multiplex quantification of indole 3-acetic acid (IAA) as well as its precursors and degradation products. As part of the protocol development, we have also established a derivatization protocol for the IAA precursors IPyA and IAAld, compounds that are highly labile during sample extraction and purification. This article describes a standard isotope dilution method for quantitative profiling of the IAA metabolome in small amounts of plant tissues. (C) 2016 by John Wiley & Sons, Inc.
Czech name
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Czech description
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Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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