Auxin metabolite profiling in isolated and intact plant nuclei
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00553347" target="_blank" >RIV/61389030:_____/21:00553347 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/21:73610751
Result on the web
<a href="http://doi.org/10.3390/ijms222212369" target="_blank" >http://doi.org/10.3390/ijms222212369</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms222212369" target="_blank" >10.3390/ijms222212369</a>
Alternative languages
Result language
angličtina
Original language name
Auxin metabolite profiling in isolated and intact plant nuclei
Original language description
The plant nucleus plays an irreplaceable role in cellular control and regulation by auxin (indole‐3‐acetic acid, IAA) mainly because canonical auxin signaling takes place here. Auxin can enter the nucleus from either the endoplasmic reticulum or cytosol. Therefore, new information about the auxin metabolome (auxinome) in the nucleus can illuminate our understanding of subcel-lular auxin homeostasis. Different methods of nuclear isolation from various plant tissues have been described previously, but information about auxin metabolite levels in nuclei is still fragmented and insufficient. Herein, we tested several published nucleus isolation protocols based on differential centrifugation or flow cytometry. The optimized sorting protocol leading to promising yield, intact-ness, and purity was then combined with an ultra‐sensitive mass spectrometry analysis. Using this approach, we can present the first complex report on the auxinome of isolated nuclei from cell cultures of Arabidopsis and tobacco. Moreover, our results show dynamic changes in auxin homeosta-sis at the intranuclear level after treatment of protoplasts with free IAA, or indole as a precursor of auxin biosynthesis. Finally, we can conclude that the methodological procedure combining flow cytometry and mass spectrometry offers new horizons for the study of auxin homeostasis at the subcellular level.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
22
Issue of the periodical within the volume
22
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
12369
UT code for WoS article
000727600500001
EID of the result in the Scopus database
2-s2.0-85118982755