The dynamics and endocytosis of Flot1 protein in response to flg22 in Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F17%3A73583635" target="_blank" >RIV/61989592:15310/17:73583635 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S017616171730130X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S017616171730130X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jplph.2017.05.010" target="_blank" >10.1016/j.jplph.2017.05.010</a>

Result language
angličtina
Original language name
The dynamics and endocytosis of Flot1 protein in response to flg22 in Arabidopsis
Original language description
Membrane microdomains play vital roles in the process of bacterial infection. The membrane microdomain associated protein Flot1 acts in an endocytic pathway and is required for seedling development, however, whether Flot1 is a part of host defense mechanisms remains unknown. During an analysis of callose deposition, we found that Flot1 amiRNAi mutants exhibited defects in response to flg22. Using variable-angle total internal reflection fluorescence microscopy (VA-TIRFM), structured illumination microscopy (SIM) and fluorescence cross spectroscopy (FCS), we determined that the dynamic behavior of GFP-Flot1 in Arabidopsis thaliana cotyledon epidermal cells changed significantly in plants treated with the elicitor flg22. Moreover, we found that Flot1 was constitutively recycled via an endocytic pathway and that flg22 could promote endocytosis. Importantly, targeting of Flot1 to the late endosome/vacuole for degradation increased in response to flg22 treatment; immunoblot analysis showed that when triggered by flg22, GFP-Flot1 was gradually degraded in a time-dependent manner. Taken together, these findings support the hypothesis that the changing of dynamics and oligomeric states can promote the endocytosis and degradation of Flot1 under flg22 treatment in plant cells.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology

Project
<a href="/en/project/GA16-24313S" target="_blank" >GA16-24313S: Revisiting microtubule organization during plant cell division, growth and morphogenesis by superresolution microscopy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)