Redox regulation of plant S-nitrosoglutathione reductase activity through post-translational modifications of cysteine residues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F17%3A73584267" target="_blank" >RIV/61989592:15310/17:73584267 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/17:00100142

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0006291X17320685" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0006291X17320685</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbrc.2017.10.090" target="_blank" >10.1016/j.bbrc.2017.10.090</a>

Result language

angličtina

Original language name

Redox regulation of plant S-nitrosoglutathione reductase activity through post-translational modifications of cysteine residues

Original language description

Nitric oxide (NO) is considered as a signalling molecule involved in a variety of important physiological and pathological processes in plant and animal systems. The major pathway of NO reactions in vivo represents S-nitrosation of thiols to form S-nitrosothiols. S-nitrosoglutathione reductase (GSNOR) is the key enzyme in the degradation pathway of S-nitrosoglutathione (GSNO), a low-molecular weight adduct of NO and glutathione. GSNOR indirectly regulates the level of protein S-nitrosothiol in the cells. This study was focused on the dynamic regulation of the activity of plant GSNORs through reversible Snitrosation and/or oxidative modifications of target cysteine residues. Pre-incubation with NO/NO- donors or hydrogen peroxide resulted in a decreased reductase and dehydrogenase activity of all studied plant GSNORs. Incubation with thiol reducing agent completely reversed inhibitory effects of nitrosative modifications and partially also oxidative inhibition. In biotin-labelled samples, S-nitrosation of plant GSNORs was confirmed after immunodetection and using mass spectrometry S-nitrosation of conserved Cys271 was identified in tomato GSNOR. Negative regulation of constitutive GSNOR activity in vivo by nitrosative or oxidative modifications might present an important mechanism to control GSNO levels, a critical mediator of the downstream signalling effects of NO, as well as for formaldehyde detoxification in dehydrogenase reaction mode.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical and Biophysical Research Communication

ISSN

0006-291X

e-ISSN

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Volume of the periodical

494

Issue of the periodical within the volume

1-2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

27-33

UT code for WoS article

000416615300006

EID of the result in the Scopus database

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