Measurement of S-Nitrosoglutathione Reductase Activity in Plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73598552" target="_blank" >RIV/61989592:15310/20:73598552 - isvavai.cz</a>
Result on the web
<a href="https://obd.upol.cz/id_publ/333178438" target="_blank" >https://obd.upol.cz/id_publ/333178438</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-9790-9_5" target="_blank" >10.1007/978-1-4939-9790-9_5</a>
Alternative languages
Result language
angličtina
Original language name
Measurement of S-Nitrosoglutathione Reductase Activity in Plants
Original language description
S-nitrosation as a redox-based posttranslational modification of protein cysteine has emerged as an integral part of signaling pathways of nitric oxide across all types of organisms. Protein S-nitrosation status is controlled by two key mechanisms: by direct denitrosation performed by the thioredoxin/thioredoxin reductase system, and in an indirect way mediated by S-nitrosoglutathione reductase (GSNOR). GSNOR, which has been identified as a key component of S-nitrosothiols catabolism, catalyzes an irreversible decomposition of abundant intracellular S-nitrosothiol, S-nitrosoglutathione (GSNO) to oxidized glutathione using reduced NADH cofactor. In plants, GSNOR has been shown to play important roles in plant growth and development and plant responses to abiotic and biotic stress stimuli. In this chapter, optimized protocols of spectrophotometric measurement of GSNOR enzymatic activity and activity staining in native polyacrylamide gels in plant GSNOR are presented.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Nitrogen Metabolism in Plants: Methods and Protocols
ISBN
978-1-4939-9789-3
Number of pages of the result
15
Pages from-to
45-59
Number of pages of the book
178
Publisher name
Humana Press
Place of publication
Clifton
UT code for WoS chapter
000560394400006