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Measurement of S-Nitrosoglutathione Reductase Activity in Plants

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73598552" target="_blank" >RIV/61989592:15310/20:73598552 - isvavai.cz</a>

  • Result on the web

    <a href="https://obd.upol.cz/id_publ/333178438" target="_blank" >https://obd.upol.cz/id_publ/333178438</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-4939-9790-9_5" target="_blank" >10.1007/978-1-4939-9790-9_5</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Measurement of S-Nitrosoglutathione Reductase Activity in Plants

  • Original language description

    S-nitrosation as a redox-based posttranslational modification of protein cysteine has emerged as an integral part of signaling pathways of nitric oxide across all types of organisms. Protein S-nitrosation status is controlled by two key mechanisms: by direct denitrosation performed by the thioredoxin/thioredoxin reductase system, and in an indirect way mediated by S-nitrosoglutathione reductase (GSNOR). GSNOR, which has been identified as a key component of S-nitrosothiols catabolism, catalyzes an irreversible decomposition of abundant intracellular S-nitrosothiol, S-nitrosoglutathione (GSNO) to oxidized glutathione using reduced NADH cofactor. In plants, GSNOR has been shown to play important roles in plant growth and development and plant responses to abiotic and biotic stress stimuli. In this chapter, optimized protocols of spectrophotometric measurement of GSNOR enzymatic activity and activity staining in native polyacrylamide gels in plant GSNOR are presented.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Nitrogen Metabolism in Plants: Methods and Protocols

  • ISBN

    978-1-4939-9789-3

  • Number of pages of the result

    15

  • Pages from-to

    45-59

  • Number of pages of the book

    178

  • Publisher name

    Humana Press

  • Place of publication

    Clifton

  • UT code for WoS chapter

    000560394400006