Cisplatin interacting with buffering media and cysteine: Molecular insight due to Raman microspectroscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73590744" target="_blank" >RIV/61989592:15310/19:73590744 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5545" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5545</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.5545" target="_blank" >10.1002/jrs.5545</a>

Result language

angličtina

Original language name

Cisplatin interacting with buffering media and cysteine: Molecular insight due to Raman microspectroscopy

Original language description

The solvation of cisplatin, [Pt(NH3)2Cl2], a widely used chemotherapeutical drug, regarded on molecular level in model media is often overlooked by researchers. It is, however, essential in several aspects. The solvation was monitored by using Raman scattering microspectroscopy. This technique being used for the first time enables us to focus the laser beam several micrometers above the dissolving crystals of cisplatin, allowing to monitor the process of cisplatin dissolution in situ. The obtained Raman microspectroscopic results provided a direct qualitative evidence about (a) interaction between Tris,MOPS, and HEPES buffers and cisplatin via Pt―N coordination covalent bond and (b) interaction of cisplatin with cysteine dominantly through Pt―S bond (at pH 7.4). Furthermore, this vibrational spectroscopic technique revealed a decreased dissolution of cisplatin in a particular buffering solution in the presence of NaCl abundance; kinetics of the Pt―N formation in the case of MOPS–NaCl medium was monitored. Moreover, spatial dissolution of cisplatin in Cys‐Tris medium was observed in situ. The particular bufferingmedium, containing either primary or tertiary amines, can interact with cisplatin via a coordination covalent bond. The presence of an increased NaCl concentration can partially suppress this interaction. Raman microspectroscopy, a method of vibrational spectroscopy, is a powerful technique to investigate the dissolution of cisplatin in situ. On the basis of vibrational microspectroscopic measurements, it provides direct evidence about cisplatin interactions with selected model media being widely used in biochemistry and biophysics. This interaction could influence the effectiveness of cisplatin as a drug.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10402 - Inorganic and nuclear chemistry

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF RAMAN SPECTROSCOPY

ISSN

0377-0486

e-ISSN

—

Volume of the periodical

50

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

528-536

UT code for WoS article

000465335700005

EID of the result in the Scopus database

2-s2.0-85059690064