Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73596541" target="_blank" >RIV/61989592:15310/19:73596541 - isvavai.cz</a>
Result on the web
<a href="https://pbsociety.org.pl/journals/index.php/aa/article/view/aa.1764/7718" target="_blank" >https://pbsociety.org.pl/journals/index.php/aa/article/view/aa.1764/7718</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5586/aa.1764" target="_blank" >10.5586/aa.1764</a>
Alternative languages
Result language
angličtina
Original language name
Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin
Original language description
The aim of the research was to establish an efficient procedure for in vitro micropropagation in order to obtain numbers of identical plants for in vitro polyploidization of Humulus lupulus (2n = 20), using antimicrotubule agent oryzalin. For this purpose, the polyploidization was carried out for H. lupulus Osvald's clones 31, 74, 114, and for 'Sladek' cultivar. The two experimental methods - the cultivation of nodal segments on medium with different concentrations of oryzalin (1, 5, and 10 mu M) for 2 weeks and the irrigation of nodal segments with oryzalin (10 and 20 mu M) for 24 and 48 h were chosen for inducing for polyploid plantlets of H. lupulus. This procedure provided tetraploids, which were identified by flow cytometry using internal standardization method and confirmed using chromosome counting of methaphasic cells from the root tips and morphological observations. The influence of chromosome doubling was also verified with stomata characterization. The polyploid plants were propagated for next evaluation, rooting and transfer to nonsterile conditions and into field. After chromosome doubling, using some different concentration of oryzalin, some plantlets became tetraploids, no mixoploids were detected. The highest efficiency of polyploidization was achieved for clone 72 after 2-week treatment of oryzalin supplemented medium. On the other hand, for method based on the irrigation of nodal segments with oryzalin, the most efficient conditions were treatment with 10 mu M and 20 mu M oryzalin for 24 and 48 h, respectively.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
<a href="/en/project/QJ1510160" target="_blank" >QJ1510160: New technologies of the gaining of bioactive compounds from medicinal and aromatic plants as a sources of active substances of botanical pesticides and food supplements.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACTA AGROBOTANICA
ISSN
0065-0951
e-ISSN
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Volume of the periodical
72
Issue of the periodical within the volume
2
Country of publishing house
PL - POLAND
Number of pages
10
Pages from-to
"1764-1"-"1764-10"
UT code for WoS article
000474433100001
EID of the result in the Scopus database
2-s2.0-85068898652