Induced polyploidization and its influence on yield, morphological, and qualitative characteristics of microtubers in Ullucus tuberosus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F12%3A43867822" target="_blank" >RIV/70883521:28110/12:43867822 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/12:00381125 RIV/60460709:41610/12:54461 RIV/00216208:11310/12:10196802
Result on the web
<a href="http://dx.doi.org/10.1007/s11240-011-0076-7" target="_blank" >http://dx.doi.org/10.1007/s11240-011-0076-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-011-0076-7" target="_blank" >10.1007/s11240-011-0076-7</a>

Result language
angličtina
Original language name
Induced polyploidization and its influence on yield, morphological, and qualitative characteristics of microtubers in Ullucus tuberosus
Original language description
This study aimed to induce in vitro polyploidization in diploid ulluco (2n = 24) using oryzalin, an antimicrotubule agent. Nodal segments were treated with 0, 20, 25 or 30 mu M oryzalin for 24 or 48 h. Ploidy determination of regenerants was based on flow cytometry and chromosome countings in metaphasic cells from root tips. After polyploidization, microtuber formation was induced in plantlets. Determination of starch content using spectrophotometric analysis, and vitamin C content by titration with 2,6-dichlorphenolindophenol were carried out to assess diploid and octoploid microtubers. Colour of in vitro plantlets was evaluated using the RHS colour chart. Out of 120 nodal segments exposed to oryzalin, 2.5% were octoploids (2n = 96). The most efficient conditions for inducing polyploidy were treatment with 20 mu M oryzalin for 24 h, followed by 25 mu M oryzalin for 48 h. No tetraploids were obtained. Moreover, flow cytometric analyses of the nuclear DNA contents revealed extensive end
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
ISSN
0167-6857
e-ISSN
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Volume of the periodical
109
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
83-90
UT code for WoS article
000301599800009
EID of the result in the Scopus database
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