Characterization of Protein Radicals in Arabidopsis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73598027" target="_blank" >RIV/61989592:15310/19:73598027 - isvavai.cz</a>
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fphys.2019.00958/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphys.2019.00958/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2019.00958" target="_blank" >10.3389/fphys.2019.00958</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of Protein Radicals in Arabidopsis
Original language description
Oxidative modification of proteins in photosystem II (PSII) exposed to high light has been studied for a few decades, but the characterization of protein radicals formed by protein oxidation is largely unknown. Protein oxidation is induced by the direct reaction of proteins with reactive oxygen species known to form highly reactive protein radicals comprising carbon-centered (alkyl) and oxygen-centered (peroxyl and alkoxyl) radicals. In this study, protein radicals were monitored in Arabidopsis exposed to high light by immuno-spin trapping technique based on the detection of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) nitrone adducts using the anti-DMPO antibody. Protein radicals were imaged in Arabidopsis leaves and chloroplasts by confocal laser scanning microscopy using fluorescein conjugated with the anti-DMPO antibody. Characterization of protein radicals by standard blotting techniques using PSII protein specific antibodies shows that protein radicals are formed on D1, D2, CP43, CP47, and Lhcb3 proteins. Protein oxidation reflected by the appearance/disappearance of the protein bands reveals that formation of protein radicals was associated with protein fragmentation (cleavage of the D1 peptide bonds) and aggregation (cross-linking with another PSII subunits). Characterization of protein radical formation is important for better understating of the mechanism of oxidative modification of PSII proteins under high light.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Physiology
ISSN
1664-042X
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
AUG
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
"958-1"-"958-10"
UT code for WoS article
000480560400001
EID of the result in the Scopus database
2-s2.0-85070554950