Determination of biogenic amines by using amperometric biosensors based on grass pea amine oxidase and oat polyamine oxidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73603549" target="_blank" >RIV/61989592:15310/20:73603549 - isvavai.cz</a>

Result on the web

<a href="http://www.chem.ubbcluj.ro/~studiachemia/issues/chemia2020_3/01_Aquilante_etal_9_21.pdf" target="_blank" >http://www.chem.ubbcluj.ro/~studiachemia/issues/chemia2020_3/01_Aquilante_etal_9_21.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.24193/subbchem.2020.3.01" target="_blank" >10.24193/subbchem.2020.3.01</a>

Result language

angličtina

Original language name

Determination of biogenic amines by using amperometric biosensors based on grass pea amine oxidase and oat polyamine oxidase

Original language description

Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by crosslinking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl 0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Studia Universitatis Babes-Bolyai Chemia

ISSN

1224-7154

e-ISSN

—

Volume of the periodical

65

Issue of the periodical within the volume

3

Country of publishing house

RO - ROMANIA

Number of pages

13

Pages from-to

9-21

UT code for WoS article

000581042000002

EID of the result in the Scopus database

2-s2.0-85092915930