Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005614" target="_blank" >RIV/61989592:15310/08:00005614 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination

Original language description

A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly selective electrochemical biosensor to a weak acid cation-exchange column for online detection of amines. A bienzyme design, based on a recently isolated amine oxidase from grass pea and commercial horseradish peroxidase, was used for the biosensor construction. The enzymes were co-immobilized on the surface of a graphite electrode together with the electrochemical mediator (Os-redox polymer). The electrochemical detection was performed at a low applied potential (-50 mV vs. Ag/AgCl, KCl0.1 M), where biases from interferences are minimal. The separation and determination of six BA, with relevance in food analysis (tyramine, putrescine, cadaverine, histamine, agmatine and spermidine), were investigated. Irrespective of the BA nature, the amine oxidase-based biosensor showed a linear response up to 5 mM, and its sensitivity decreases in the following order: cada

Czech name

Amperometrický biosenzor aminooxidasy spojený s kapalinovou chromatografii pro determinaci aminů

Czech description

Byla připravená selektivní metoda pro stanovení biogenních aminů. Využilo se biosenzoru s navázanou aminooxidasou napojeného na kolonu HPLC. Metoda měla velice nízký práh detekce a lze ji využít k určování čerstvosti ryb.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microchimica Acta

ISSN

0026-3672

e-ISSN

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Volume of the periodical

163

Issue of the periodical within the volume

3-4

Country of publishing house

AT - AUSTRIA

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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