Influence of Membrane Phase on the Optical Properties of DPH
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73603973" target="_blank" >RIV/61989592:15310/20:73603973 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1420-3049/25/18/4264/htm" target="_blank" >https://www.mdpi.com/1420-3049/25/18/4264/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules25184264" target="_blank" >10.3390/molecules25184264</a>
Alternative languages
Result language
angličtina
Original language name
Influence of Membrane Phase on the Optical Properties of DPH
Original language description
The fluorescent molecule diphenylhexatriene (DPH) has been often used in combination with fluorescence anisotropy measurements, yet little is known regarding the non-linear optical properties. In the current work, we focus on them and extend the application to fluorescence, while paying attention to the conformational versatility of DPH when it is embedded in different membrane phases. Extensive hybrid quantum mechanics/molecular mechanics calculations were performed to investigate the influence of the phase- and temperature-dependent lipid environment on the probe. Already, the transition dipole moments and one-photon absorption spectra obtained in the liquid ordered mixture of sphingomyelin (SM)-cholesterol (Chol) (2:1) differ largely from the ones calculated in the liquid disordered DOPC and solid gel DPPC membranes. Throughout the work, the molecular conformation in SM:Chol is found to differ from the other environments. The two-photon absorption spectra and the ones obtained by hyper-Rayleigh scattering depend strongly on the environment. Finally, a stringent comparison of the fluorescence anisotropy decay and the fluorescence lifetime confirm the use of DPH to gain information upon the surrounding lipids and lipid phases. DPH might thus open the possibility to detect and analyze different biological environments based on its absorption and emission properties.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MOLECULES
ISSN
1420-3049
e-ISSN
—
Volume of the periodical
25
Issue of the periodical within the volume
18
Country of publishing house
CH - SWITZERLAND
Number of pages
20
Pages from-to
"4264-1"-"4264-20"
UT code for WoS article
000581844900001
EID of the result in the Scopus database
2-s2.0-85091166253