The nuclear GUCT domain‑containing DEAD‑box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73607714" target="_blank" >RIV/61989592:15310/21:73607714 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11103-021-01152-w" target="_blank" >https://link.springer.com/article/10.1007/s11103-021-01152-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11103-021-01152-w" target="_blank" >10.1007/s11103-021-01152-w</a>
Alternative languages
Result language
angličtina
Original language name
The nuclear GUCT domain‑containing DEAD‑box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens.
Original language description
The DEAD-box-containing RNA helicase family encompasses a large and functionally important group of enzymes involved in cellular processes committed to the metabolism of RNA, including its transcription, processing, transport, translation and decay. Studies indicate this protein family has implied roles in plant vegetative and reproductive developmental processes as well as response to environmental stresses such has cold and high salinity. We focus here on a small conserved sub-group of GUCT domain-containing RNA helicase in the moss Physcomitrium patens. Phylogenetic analysis shows that RNA helicases containing the GUCT domain form a distinct conserved clade across the green lineage. In this clade, the P. patens genome possesses two closely related paralogues RNA helicases predicted to be nuclear, PpRH1 and PpRH2. Using in-locus gene fluorescent tagging we show that PpRH1 is localized to the nucleus in protonema. Analysis of PpRH1 and PpRH2 deletions, individually and together, indicates their potential roles in protonema, gametophore and sporophyte cellular and tissue development in P. patens. Additionally, the ultrastructural analysis of phyllid chloroplasts in Δrh2 and Δrh1/2 shows distinct starch granule accumulation under standard growth conditions associated with changes in photosynthetic activity parameters. We could not detect effects of either temperature or stress on protonema growth or PpRH1 and PpRH2 expression. Together, these results suggest that nuclear GUCT-containing RNA helicases play a role primarily in developmental processes directly or indirectly linked to photosynthesis activity in the moss P. patens.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT MOLECULAR BIOLOGY
ISSN
0167-4412
e-ISSN
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Volume of the periodical
107
Issue of the periodical within the volume
4-5
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
19
Pages from-to
307-325
UT code for WoS article
000642394800001
EID of the result in the Scopus database
2-s2.0-85105158820