Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00532865" target="_blank" >RIV/61389030:_____/20:00532865 - isvavai.cz</a>
Result on the web
<a href="http://doi.org/10.1016/j.envexpbot.2020.104236" target="_blank" >http://doi.org/10.1016/j.envexpbot.2020.104236</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envexpbot.2020.104236" target="_blank" >10.1016/j.envexpbot.2020.104236</a>
Alternative languages
Result language
angličtina
Original language name
Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
Original language description
One of the early reactions of plants to high levels of NaCl is the generation of reactive oxygen species (ROS), which is critical for stress signaling, Na+ extrusion, oxidative damage and programmed cell death (PCD) under salt stress. Using moss Physcomitrella patens protonema as a model system, we have tested whether ROS generation and DNA degradation can be induced by salinity in Bryophyta. We have shown with dihydroethidium in vivo test that toxic NaCl concentrations significantly stimulate ROS production. The pharmacological analysis carried out using superoxide dismutase, catalase, dimethyl sulfoxide, thiourea and spermine outlines the superoxide and hydroxyl radicals as major NaCl-induced species. Using Comet assay, we have found that NaCl affects the DNA stability in ROS-dependent manner, increasing both single and double strand DNA breaks (SSBs and DSBs, respectively). Surprisingly, DSBs were induced by NaCl to a lesser extent than SSBs. This is indicative of an unexpected and sophisticated action of NaCl on moss genome including DSBs through PCD and SSBs caused by hydroxyl radical-induced DNA oxidation or transposon rearrangement. We also report a relatively high sensitivity of Physcomitrella patens protonema to NaCl compared to later developmental stages of this moss, suggesting that dividing cells are more sensitive to NaCl than other tissues.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LTC17047" target="_blank" >LTC17047: The use of plants in monitoring of human DNA damage.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental and Experimental Botany
ISSN
0098-8472
e-ISSN
—
Volume of the periodical
180
Issue of the periodical within the volume
December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
104236
UT code for WoS article
000579447700008
EID of the result in the Scopus database
2-s2.0-85090573884