Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00423951" target="_blank" >RIV/61388963:_____/13:00423951 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/13:00423951

Result on the web

<a href="http://dx.doi.org/10.1155/2013/535049" target="_blank" >http://dx.doi.org/10.1155/2013/535049</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2013/535049" target="_blank" >10.1155/2013/535049</a>

Result language

angličtina

Original language name

Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

Original language description

The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and double strand breaks in wild type and mutant lig4 Physcomitrella lines. Moreover, APT gene when inactivated by induced mutations was used as selectable marker to ascertain mutational background at nucleotide level by sequencing of the APT locus. We show that extensive repair of DSBs occurs also in the absence of the functional LIG4, whereas repair of SSBs is seriously compromised. From analysis of induced mutations we conclude that their accumulation rather than remaining lesions in DNA and blocking progression through cell cycle is incompatible with normal plant growth and development and leads to s

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LD13006" target="_blank" >LD13006: Molecular basis of plant response to UV-B radiation</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMED RESEARCH INTERNATIONAL

ISSN

2314-6133

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

ID 535049

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

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UT code for WoS article

000328834200001

EID of the result in the Scopus database

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