Mutagenesis during plant responses to UVB radiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00447257" target="_blank" >RIV/61389030:_____/15:00447257 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.plaphy.2014.12.013" target="_blank" >http://dx.doi.org/10.1016/j.plaphy.2014.12.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2014.12.013" target="_blank" >10.1016/j.plaphy.2014.12.013</a>

Result language

angličtina

Original language name

Mutagenesis during plant responses to UVB radiation

Original language description

We tested an idea that induced mutagenesis due to unrepaired DNA lesions, here the UV photoproducts, underlies the impact of UVB irradiation on plant phenotype. For this purpose we used protonemal culture of the moss Physcomitrella patens with 50% of apical cells, which mimics actively growing tissue, the most vulnerable stage for the induction of mutations. We measured the UVB mutation rate of various moss lines with defects in DNA repair (pplig4, ppku70, pprad50, ppmre11), and in selected clones resistant to 2-Fluoroadenine, which were mutated in the adenosine phosphotrasferase gene (APT), we analysed induced mutations by sequencing. In parallel we followed DNA break repair and removal of cyclobutane pyrimidine dimers with a half-life tau = 4 h 14 min determined by comet assay combined with UV dimer specific T4 endonuclease V. We show that UVB induces massive, sequence specific, error-prone bypass repair that is responsible for a high mutation rate owing to relatively slow, though er

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/LD13006" target="_blank" >LD13006: Molecular basis of plant response to UV-B radiation</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

—

Volume of the periodical

93

Issue of the periodical within the volume

SI

Country of publishing house

FR - FRANCE

Number of pages

5

Pages from-to

29-33

UT code for WoS article

000357223100005

EID of the result in the Scopus database

—