Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73608446" target="_blank" >RIV/61989592:15310/21:73608446 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2227-9040/9/6/119/htm" target="_blank" >https://www.mdpi.com/2227-9040/9/6/119/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/chemosensors9060119" target="_blank" >10.3390/chemosensors9060119</a>

Result language
angličtina
Original language name
Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection
Original language description
A new concept for simultaneous detection of two model proteases based on immobilized fluorescently labelled peptides was developed and evaluated. Each probe was composed of a carrier modified by poly(ethylene glycol) (PEG) chains, a specifically cleavable linker, and a fluorescent dye incorporated in the peptide tail. Based on a single excitation-double emission fluorescence response of liberated fluorophores caused by enzymatic cleavage, the presence of a single or both proteases in a mixture was unambiguously determined in an experimentally established concentration range. Among the tested solid supports, Rink Amide PEGA resin was recognized as the most suitable choice from the perspective of on-resin enzyme assays.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10401 - Organic chemistry

Project
<a href="/en/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescent probes responding to hydrolases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)