Fluorescence-Based On-Resin Detection of ThreeModel Proteases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73609992" target="_blank" >RIV/61989592:15310/21:73609992 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2227-9040/9/12/359/htm" target="_blank" >https://www.mdpi.com/2227-9040/9/12/359/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/chemosensors9120359" target="_blank" >10.3390/chemosensors9120359</a>
Alternative languages
Result language
angličtina
Original language name
Fluorescence-Based On-Resin Detection of ThreeModel Proteases
Original language description
A new approach to on-resin detection of three model proteases (trypsin, chymotrypsin, and thrombin) has been developed, while at the same time already described methodology for simultaneous detection of two enzymes (trypsin and chymotrypsin) has been additionally generalized. Appropriate immobilized substrates, comprising specifically cleavable peptide sequences capped with fluorescent dyes, have been synthesized on Rink Amide PEGA resin or Amino PEGA resin modified with backbone amide linker (BAL). Resulting solid support-bound probes were then dispersed into Tris-HCl buffer solution (pH = 8.0) and subjected to enzymatic cleavage. Liberated fluorophores have been tracked by fluorescence measuring. The competitive activities of studied proteases towards the thrombin probe have been efficiently limited and controlled by employing a Bowman-Birk inhibitor into a system.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
<a href="/en/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescent probes responding to hydrolases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemosensors
ISSN
2227-9040
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
"359-1"-"359-11"
UT code for WoS article
000743064700001
EID of the result in the Scopus database
2-s2.0-85121827980