In vitro cytotoxic effects of secondary metabolites present in Sarcopoterium Spinosum L.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73609997" target="_blank" >RIV/61989592:15310/21:73609997 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2076-3417/11/11/5300/htm" target="_blank" >https://www.mdpi.com/2076-3417/11/11/5300/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app11115300" target="_blank" >10.3390/app11115300</a>

Result language

angličtina

Original language name

In vitro cytotoxic effects of secondary metabolites present in Sarcopoterium Spinosum L.

Original language description

arcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with Et acetate and in its sub-fraction obtained by column chromatog. three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by NMR (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatog. (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10-7 M) and MCF-7 cells (IC50 5.10-6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the Et acetate extract can exert a significant dose dependent in vitro cytotoxicity.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10401 - Organic chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Sciences-Basel

ISSN

2076-3417

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

"5300-1"-"5300-11"

UT code for WoS article

000659567600001

EID of the result in the Scopus database

2-s2.0-85108526661