Lycopene beta-cyclase expression influences plant physiology, development, and metabolism in tobacco plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73610444" target="_blank" >RIV/61989592:15310/21:73610444 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jxb/article/72/7/2544/6116658" target="_blank" >https://academic.oup.com/jxb/article/72/7/2544/6116658</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erab029" target="_blank" >10.1093/jxb/erab029</a>
Alternative languages
Result language
angličtina
Original language name
Lycopene beta-cyclase expression influences plant physiology, development, and metabolism in tobacco plants
Original language description
Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. beta-Carotene is generated from lycopene by lycopene beta-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco (Nicotiana tabacum cv. Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased and their leaf GA1 content was reduced. RNA interference (RNAi) NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and light-saturated capacity of linear electron transport) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue these phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial or detrimental for plant growth.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA18-10349S" target="_blank" >GA18-10349S: Gibberellin biosynthesis and signal transduction – identification of novel targets for plant growth regulation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF EXPERIMENTAL BOTANY
ISSN
0022-0957
e-ISSN
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Volume of the periodical
72
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
26
Pages from-to
2544-2569
UT code for WoS article
000639080100023
EID of the result in the Scopus database
2-s2.0-85104827778