Triple-FRET multi-purpose fluorescent probe for three-protease detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73615737" target="_blank" >RIV/61989592:15310/22:73615737 - isvavai.cz</a>
Result on the web
<a href="https://pubs.rsc.org/en/content/articlepdf/2022/ra/d2ra05125g" target="_blank" >https://pubs.rsc.org/en/content/articlepdf/2022/ra/d2ra05125g</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d2ra05125g" target="_blank" >10.1039/d2ra05125g</a>

Result language
angličtina
Original language name
Triple-FRET multi-purpose fluorescent probe for three-protease detection
Original language description
A new, robust and reliable methodology for three-protease screening in a single-enzyme mode has been developed and verified, employing a multi-purpose peptide probe with three selectively cleavable sites furnished with four fluorophores. A triple-FRET-based single-excitation quadruple-emission concept for unambiguous sensing of trypsin, chymotrypsin and caspase-8 in the lowest detectable concentrations of 0.5 ng mL(-1), 0.2 mu g mL(-1), and 2 U mL(-1), respectively, has been applied and graphically depicted. Then the developed 4-dye probe has been also studied from the perspective of simultaneous two-protease screening, which was found only partially feasible, primarily due to unselective chymotrypsin cleavage.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10401 - Organic chemistry

Project
<a href="/en/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescent probes responding to hydrolases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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