Modern Comprehensive Metabolomic Profiling of Pollen Using Various Analytical Techniques
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73633554" target="_blank" >RIV/61989592:15310/25:73633554 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1420-3049/30/5/1172" target="_blank" >https://www.mdpi.com/1420-3049/30/5/1172</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules30051172" target="_blank" >10.3390/molecules30051172</a>
Alternative languages
Result language
angličtina
Original language name
Modern Comprehensive Metabolomic Profiling of Pollen Using Various Analytical Techniques
Original language description
Pollen is a cornerstone of life for plants. Its durability, adaptability, and complex design are the key factors to successful plant reproduction, genetic diversity, and the maintenance of ecosystems. A detailed study of its chemical composition is important to understand the mechanism of pollen-pollinator interactions, pollination processes, and allergic reactions. In this study, a multimodal approach involving Fourier transform infrared spectrometry (FTIR), direct mass spectrometry with an atmospheric solids analysis probe (ASAP), matrix-assisted laser desorption/ionization (MALDI) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) was applied for metabolite profiling. ATR-FTIR provided an initial overview of the present metabolite classes. Phenylpropanoid, lipidic, and carbohydrate structures were revealed. The hydrophobic outer layer of pollen was characterized in detail by ASAP-MS profiling, and esters, phytosterols, and terpenoids were observed. Diacyl- and triacylglycerols and carbohydrate structures were identified in MALDI-MS spectra. The MALDI-MS imaging of lipids proved to be helpful during the microscopic characterization of pollen species in their mixture. Polyphenol profiling and the quantification of important secondary metabolites were performed by UHPLC-MS in context with pollen coloration and their antioxidant and antimicrobial properties. The obtained results revealed significant chemical differences among Magnoliophyta and Pinophyta pollen. Additionally, some variations within Magnoliophyta species were observed. The obtained metabolomics data were utilized for pollen differentiation at the taxonomic scale and provided valuable information in relation to pollen interactions during reproduction and its related applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA24-10730S" target="_blank" >GA24-10730S: Hilum - the seeds Achilles heel? The enigma of hilum formation and polyphenol oxidase function in seeds.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MOLECULES
ISSN
1420-3049
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
22
Pages from-to
"1172-1"-"1172-22"
UT code for WoS article
001442676700001
EID of the result in the Scopus database
2-s2.0-86000577977