Cytokinin oxidase (or cytokinin dehydrogenase?) from wheat: Purification, characterization and evidence for dehydrogenase mechanism of the enzyme reaction.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F99%3A00000767" target="_blank" >RIV/61989592:15310/99:00000767 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cytokinin oxidase (or cytokinin dehydrogenase?) from wheat: Purification, characterization and evidence for dehydrogenase mechanism of the enzyme reaction.
Original language description
The key enzyme in the degradation of plant hormones cytokinins, generally termed cytokinin oxidase, was purified to homogeneity from swollen wheat grains (Triticum aestivum) by a multistep procedure including low pressure chromatography, preparative electrophoresis and extensive FPLC separations resulting in a purification degree over 3,300-fold. New techniques were developed for the enzyme activity assay and staining on native electrophoretic gels to confirm the identity of the protein. The enzyme wasobtained with the highest specific activity ever reported, 44 nkat/mg. Purified enzyme is a monomeric flavoprotein of 39 kDa. The enzyme catalyzes the cleavage of N6-unsaturated isoprenoid side chain of cytokinins, preferably with a hydrophilic group inposition 2 of the purine ring, while the compounds with aromatic side chains on N6 are inhibitors. Contrary to the generally accepted view, it was found that oxygen is not required for the catalytic reaction and hydrogen peroxide is not p
Czech name
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Czech description
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Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
International Symposium on Auxins and cytokinins in plant development, Book of Abstracts (Biol. Plantarum, Vol. 42)
ISBN
0006-3134
Number of pages of the result
122
Pages from-to
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Number of pages of the book
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Publisher name
Česká akademie věd
Place of publication
Praha
UT code for WoS chapter
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