High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001414" target="_blank" >RIV/61989592:15410/03:00001414 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices

Original language description

HPLC and CE methods were employed to determine the quaternary benzo[c]phenanthridine alkaloid sanguinarine,in biological matrices (rat hepatocytes, human gingival fibroblasts, feed, porcine faeces, body fluids and tissues). HPLC was carried out on a C-18column using gradient elution and ion pairing techniques with 1-heptanesulfonic acid as ion pairing agent under acidic conditions. The detection limit for fluorimetric detection at lambda(ex) = 327 nm and lambda(em) = 577 nm was 3 nM sanguinarine. CE analyses were performed in 50 mM phosphate-Na buffer pH 2.5, with 150 mM SDS used for pre-concentration by the sweeping effect. This experimental configuration allows injecting the total capillary length with sanguinarine sample. The detection limit for UVdetection at 285 nm was 12 nM. Both methods are suitable for analysing submicromolar quantities of sanguinarine in biological materials. The HPLC method is more sensitive than CE because it uses fluorescence detection.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

679-685

UT code for WoS article

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EID of the result in the Scopus database

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