Chemiluminescence as a tool for detection of reactive oxygen species - the employment of Cypridina luciferin analogues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001526" target="_blank" >RIV/61989592:15410/03:00001526 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Chemiluminescence as a tool for detection of reactive oxygen species - the employment of Cypridina luciferin analogues

Original language description

Luminol, CLA and MCLA CL were studied in three ROS generating chemical systems in vitro (horseradish peroxidase-H2O2, CuSO4-H2O2 and NaClO-H2O2 system). Measurements were carried out in phosphate buffer (pH 7.4) at 25°C and 37°C using luminometer Fluoroskan Ascent. Superoxide dismutase and sodium azide were used as specific scavenger of superoxide anion and quencher of singlet oxygen, respectively. Their effect was compared with that of Trolox (water soluble analogue of vitamin E), that should scavengeall reactive species present in the reaction mixture.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemické listy

ISSN

0009-2770

e-ISSN

—

Volume of the periodical

97

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

1

Pages from-to

1132

UT code for WoS article

—

EID of the result in the Scopus database

—