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Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73604523" target="_blank" >RIV/61989592:15640/21:73604523 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15310/21:73604523

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S187167842030193X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S187167842030193X</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.nbt.2020.11.003" target="_blank" >10.1016/j.nbt.2020.11.003</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production

  • Original language description

    The parasitic fungus Claviceps purpurea has been used for decades by the pharmaceutical industry as a valuable producer of ergot alkaloids. As the biosynthetic pathway of ergot alkaloids involves a common precursor L-tryptophan, targeted genetic modification of the related genes may improve production yield. In this work, the S76L mutated version of the trpE gene encoding anthranilate synthase was constitutively overexpressed in the fungus with the aim of overcoming feedback inhibition of the native enzyme by an excess of tryptophan. In another approach, the dmaW gene encoding dimethylallyltryptophan synthase, which produces a key intermediate for the biosynthesis of ergot alkaloids, was also constitutively overexpressed. Each of the above manipulations led to a significant increase (up to 7-fold) in the production of ergot alkaloids in submerged cultures.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

  • Continuities

    O - Projekt operacniho programu

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    New Biotechnology

  • ISSN

    1871-6784

  • e-ISSN

  • Volume of the periodical

    61

  • Issue of the periodical within the volume

    MAR

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

    69-79

  • UT code for WoS article

    000606593400009

  • EID of the result in the Scopus database

    2-s2.0-85096827896