Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73604523" target="_blank" >RIV/61989592:15640/21:73604523 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/21:73604523
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S187167842030193X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S187167842030193X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nbt.2020.11.003" target="_blank" >10.1016/j.nbt.2020.11.003</a>
Alternative languages
Result language
angličtina
Original language name
Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production
Original language description
The parasitic fungus Claviceps purpurea has been used for decades by the pharmaceutical industry as a valuable producer of ergot alkaloids. As the biosynthetic pathway of ergot alkaloids involves a common precursor L-tryptophan, targeted genetic modification of the related genes may improve production yield. In this work, the S76L mutated version of the trpE gene encoding anthranilate synthase was constitutively overexpressed in the fungus with the aim of overcoming feedback inhibition of the native enzyme by an excess of tryptophan. In another approach, the dmaW gene encoding dimethylallyltryptophan synthase, which produces a key intermediate for the biosynthesis of ergot alkaloids, was also constitutively overexpressed. Each of the above manipulations led to a significant increase (up to 7-fold) in the production of ergot alkaloids in submerged cultures.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
O - Projekt operacniho programu
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Biotechnology
ISSN
1871-6784
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
MAR
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
69-79
UT code for WoS article
000606593400009
EID of the result in the Scopus database
2-s2.0-85096827896