Multifunctional 3D Hierarchical Bioactive Green Carbon-Based Nanocomposites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73612400" target="_blank" >RIV/61989592:15640/21:73612400 - isvavai.cz</a>
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acssuschemeng.1c00781" target="_blank" >https://pubs.acs.org/doi/10.1021/acssuschemeng.1c00781</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acssuschemeng.1c00781" target="_blank" >10.1021/acssuschemeng.1c00781</a>
Alternative languages
Result language
angličtina
Original language name
Multifunctional 3D Hierarchical Bioactive Green Carbon-Based Nanocomposites
Original language description
Carbon-based nanocarriers such as multiwall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) have shown promising delivery capabilities due to their low immunogenicity, superior internalization, and suitable cell penetration efficiency. Herein, a molecular engineering strategy is advanced for the one-pot synthesized rGO/MWCNT/Fe3O4/ZnO to enhance the stability of the nanocarrier in the biological matrix; green synthesized ZnO was responsible for water uptake and reduced cytotoxicity, while Fe3O4 controlled the cellular internalization for gene delivery. Surface morphology of the ensuing nanocomposite was correlated with photocatalytic and gene delivery (CRISPR/Cas9) features. For the first time, a complete physical interaction between CRISPR/Cas9 and nanomaterial is evidenced via atomic force microscopy (AFM), demonstrating an increase in green fluorescence protein (EGFP) up to 11%. Furthermore, the enhanced photocatalytic activity is displayed in complete degradation of the methylene blue dye under 10 min with an efficiency of over 98%. The cytotoxicity of the nanocomposite is enhanced by ZnO on treatment with the PC12 and HEK-293 cell lines subsequent to 24, 48, and 72 h of exposure, with more than 88, 79, and 80% cell viabilities for PC12 and more than 88, 80, and 85% cell viabilities for HEK-293 in the maximum ratio of material to CRISPR (WR of nM/CC being 100). Furthermore, the nanocomposite showed an antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria (MZI values of 24 and 21 mm, respectively). The surface chemistry of the optimized system opens up new prospects to codeliver therapeutic agents for useful clinical applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Sustainable Chemistry & Engineering
ISSN
2168-0485
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
26
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
8706-8720
UT code for WoS article
000671060400005
EID of the result in the Scopus database
2-s2.0-85110274447