Enhancement of thermostability of aspergillus flavus urate oxidase by immobilization on the ni-based magnetic metal–organic framework

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73612401" target="_blank" >RIV/61989592:15640/21:73612401 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2079-4991/11/7/1759/htm" target="_blank" >https://www.mdpi.com/2079-4991/11/7/1759/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/nano11071759" target="_blank" >10.3390/nano11071759</a>

Result language

angličtina

Original language name

Enhancement of thermostability of aspergillus flavus urate oxidase by immobilization on the ni-based magnetic metal–organic framework

Original language description

The improvement in the enzyme activity of Aspergillus flavus urate oxidase (Uox) was attained by immobilizing it on the surface of a Ni-based magnetic metal-organic framework (NimMOF) nanomaterial; physicochemical properties of NimMOF and its application as an enzyme stabilizing support were evaluated, which revealed a significant improvement in its stability upon immobilization on NimMOF (Uox@NimMOF). It was affirmed that while the free Uox enzyme lost almost all of its activity at similar to 40-45 degrees C, the immobilized Uox@NimMOF retained around 60% of its original activity, even retaining significant activity at 70 degrees C. The activation energy (Ea) of the enzyme was calculated to be similar to 58.81 kJ mol(-1) after stabilization, which is approximately half of the naked Uox enzyme. Furthermore, the external spectroscopy showed that the MOF nanomaterials can be coated by hydrophobic areas of the Uox enzyme, and the immobilized enzyme was active over a broad range of pH and temperatures, which bodes well for the thermal and long-term stability of the immobilized Uox on NimMOF.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanomaterials

ISSN

2079-4991

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

20

Pages from-to

"nečíslováno"

UT code for WoS article

000676558000001

EID of the result in the Scopus database

2-s2.0-85108994034