Recent advances in molecular farming using monocot plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F22%3A73618621" target="_blank" >RIV/61989592:15640/22:73618621 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S073497502200009X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S073497502200009X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biotechadv.2022.107913" target="_blank" >10.1016/j.biotechadv.2022.107913</a>

Result language

angličtina

Original language name

Recent advances in molecular farming using monocot plants

Original language description

Heterologous synthesis of proteins or peptides in plant-based systems, referred to as plant molecular farming, is a practical and safe approach for the large-scale and cost-effective production of therapeutic biomolecules. In this context, monocotyledonous plants, and especially cereals, have been considered attractive vehicles for producing high-value recombinant proteins. The endosperm, as the largest grain storage compartment, offers an appropriate environment for long-lasting protein accumulation. During the last decades, fascinating progress has been achieved in the gene transfer technology and genetic manipulation of the monocot crops using either Agrobacterium tumefaciens or direct gene transfer by biolistic methods. Our group has recently expressed biologically active recombinant human peptide cathelicidin in barley grains using endosperm-specific promoter and brought such engineered lines to field cultivation under current EU regulations for genetically modified organisms. This article reviews the most recent advances and strategies for the production of biopharmaceutical proteins in transgenic monocots, highlighting various aspects involved in recombinant protein accumulation in grains, and discussing current bottlenecks and perspectives for the biosynthesis of therapeutic molecules using different monocot plant platforms.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10620 - Other biological topics

Project

<a href="/en/project/EF17_048%2F0007323" target="_blank" >EF17_048/0007323: Development of pre-applied research in nanotechnogy and biotechnology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOTECHNOLOGY ADVANCES

ISSN

0734-9750

e-ISSN

1873-1899

Volume of the periodical

58

Issue of the periodical within the volume

September

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

"nečíslováno"

UT code for WoS article

000830105100006

EID of the result in the Scopus database

2-s2.0-85123676608