Nanoscale Topography of Anodic TiO2 Nanostructures Is Crucial for Cell-Surface Interactions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F24%3A73624734" target="_blank" >RIV/61989592:15640/24:73624734 - isvavai.cz</a>

Result on the web

<a href="https://pubs.acs.org/doi/10.1021/acsami.3c16033" target="_blank" >https://pubs.acs.org/doi/10.1021/acsami.3c16033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.3c16033" target="_blank" >10.1021/acsami.3c16033</a>

Result language

angličtina

Original language name

Nanoscale Topography of Anodic TiO2 Nanostructures Is Crucial for Cell-Surface Interactions

Original language description

Anodic titanium dioxide (TiO2 ) nanostructures, i.e., obtained by electrochemical anodization, have excellent control over the nanoscale morphology and have been extensively investigated in biomedical applications owing to their sub-100 nm nanoscale topography range and beneficial effects on biocompatibility and cell interactions. Herein, we obtain TiO2 nanopores (NPs) and nanotubes (NTs) with similar morphologies, namely, 15 nm diameter and 500 nm length, and investigate their characteristics and impact on stem cell adhesion. We show that the transition of TiO2 NPs to NTs occurs via a pore/wall splitting mechanism and the removal of the fluoride-rich layer. Furthermore, in contrast to the case of NPs, we observe increased cell adhesion and proliferation on nanotubes. The enhanced mesenchymal stem cell adhesion/proliferation seems to be related to a 3-fold increase in activated integrin clustering, as confirmed by immunogold labeling with beta 1 integrin antibody on the nanostructured layers. Moreover, computations of the electric field and surface charge density show increased values at the inner and outer sharp edges of the top surfaces of the NTs, which in turn can influence cell adhesion by increasing the bridging interactions mediated by proteins and molecules in the environment. Collectively, our results indicate that the nanoscale surface architecture of the lateral spacing topography can greatly influence stem cell adhesion on substrates for biomedical applications.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACS Applied Materials &amp; Interfaces

ISSN

1944-8244

e-ISSN

1944-8252

Volume of the periodical

16

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

"4430 "- 4438

UT code for WoS article

001155531100001

EID of the result in the Scopus database

2-s2.0-85183521722