Cellular Effects of Cationic Copper(II) Schiff Base Complexes: Anti-Inflammatory and Antiproliferative Properties
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F24%3A73625492" target="_blank" >RIV/61989592:15640/24:73625492 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/24:73625492
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cmdc.202400214" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cmdc.202400214</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cmdc.202400214" target="_blank" >10.1002/cmdc.202400214</a>
Alternative languages
Result language
angličtina
Original language name
Cellular Effects of Cationic Copper(II) Schiff Base Complexes: Anti-Inflammatory and Antiproliferative Properties
Original language description
A series of potassium isothiocyanato-(N-salicylidene-aminoacidato) cuprates (1-5) with the general formula of the monomeric unit K[Cu(sal-aa)(NCS)] & sdot; xH(2)O (x=0 or 2), containing a Schiff-base ligand (H(2)sal-aa) derived from natural amino acids such as glycine, DL-alpha-alanine, DL-valine, DL-phenylalanine and beta-alanine, and salicylaldehyde, was screened for in vitro antiradical and major cellular effects against selected cancerous and normal cells. The complexes exhibited strong antioxidant properties against superoxide in vitro and a protective effect on DNA under Fenton-like reaction conditions. Screening of their cellular effects revealed moderate in vitro cytotoxicity against human cancer cell lines (A2780, A2780R and MCF-7), with IC50 values of 25-35 mu M, and relatively low toxicity to normal fibroblast MRC-5 cells (with IC50 values>50 mu M). Additional experiments performed on A2780 cells revealed that the most potent complex 5 significantly increased the number of A2780 cells arrested in the G2/M phase of the cell cycle and triggered intracellular oxidative stress. The selected flow cytometry experiments (detection of apoptosis/autophagy and activation of caspases 3/7 and depletion of mitochondrial membrane potential) did not reveal the dominant mechanism underlying the cytotoxicity of the complexes but clearly differentiated their molecular effects from those of the reference drug cisplatin. All the complexes exerted anti-inflammatory effects by modulating the levels of the proinflammatory cytokines TNF-alpha and IL-1 beta in LPS-activated THP-1 macrophage-like cells. Complex 5 also slightly influenced the activity of the upstream NF-kappa B transcription factor, while no effect on PPAR gamma activation was detected.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
<a href="/en/project/GF21-38204L" target="_blank" >GF21-38204L: Complexes of selected transition metals with plant-derived compounds with anti-NF-kappa B and pro-PPAR dual activities</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemMedChem
ISSN
1860-7179
e-ISSN
1860-7187
Volume of the periodical
19
Issue of the periodical within the volume
19
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
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UT code for WoS article
001287592600001
EID of the result in the Scopus database
2-s2.0-85200770251