Aggregation and Other Intermolecular Interactions of Biological Buffers Observed by Capillary Electrophoresis and UV Photometry.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F04%3A00000839" target="_blank" >RIV/62156489:43210/04:00000839 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/04:00102293 RIV/61989592:15310/04:00009937

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Aggregation and Other Intermolecular Interactions of Biological Buffers Observed by Capillary Electrophoresis and UV Photometry.

Original language description

Mobilities and shapes of zones of sanguinarine and chelerythrine around pH 7 are affected by very low solubility of uncharged pseudobases of sanguinarine and chelerythrine in water and by pH-dependent solubility of their charged quaternary form. The solubility of the sanguinarine pseudobase in water is a few micromols at the most and total sanguinarine solubility in MOPS pH 7.4 was estimated to 50 uM. Sanguinarine zones in this buffer are tailed peaks with mobility independent of the injected sanguinarine concentration if it is close to 50 uM or lower. The chelerythrine solubility is higher because of the lower dissociation constant of chelerythrine cations. Precipitation of quaternary cations of sanguinarine and chelerythrine with the phosphate anionsdecelerates their electrophoretic transport in phospahte buffers and enhances the peak tailing. Sanguinarine solubility is 5 microM at the most in 13 mM sodium phosphate buffer pH 7.4. Increasing of the sample pH up to 3 decreases the ta

Czech name

Agregace a další mezimolekulární interakce biologických pufrů pozorované pomocí v kapilární elketroforézy

Czech description

Mobilities and shapes of zones of sanguinarine and chelerythrine around pH 7 are affected by very low solubility of uncharged pseudobases of sanguinarine and chelerythrine in water and by pH-dependent solubility of their charged quaternary form. The solubility of the sanguinarine pseudobase in water is a few micromols at the most and total sanguinarine solubility in MOPS pH 7.4 was estimated to 50 uM. Sanguinarine zones in this buffer are tailed peaks with mobility independent of the injected sanguinarine concentration if it is close to 50 uM or lower. The chelerythrine solubility is higher because of the lower dissociation constant of chelerythrine cations. Precipitation of quaternary cations of sanguinarine and chelerythrine with the phosphate anionsdecelerates their electrophoretic transport in phospahte buffers and enhances the peak tailing. Sanguinarine solubility is 5 uM at the most in 13 mM sodium phosphate buffer pH 7.4. Increasing of the sample pH up to 3 decreases the tailin

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Volume of the periodical

1051

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

75-84

UT code for WoS article

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EID of the result in the Scopus database

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