Determination of thiol compounds using high-performance liquid chromatography with electrochemical detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F04%3A00001693" target="_blank" >RIV/62156489:43210/04:00001693 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of thiol compounds using high-performance liquid chromatography with electrochemical detection

Original language description

Thiol compounds such as cystein (Cys), reduced (GSH) and oxidized (GSSG) glutathione and phytochelatins (PCs) play an important role in heavy metal detoxification in plants. These thiols are biological active compounds whose function is elimination of oxidative stress in plant cells. The aim of our work was to optimise sensitive and rapid method of high-performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of abovementioned thiol compounds in biological samples. An HPLC-ED system was consisted of two delivery pumps, chromatographic column Zorbax XDB (150.0 x 2.1 mm, 5 um particle size) and CoulArray detector containing eight working electrodes made from porous graphite. The most suitable isocratic mobile phase for the determination and separation of Cys, GSH, GSSG and PC2 consisted of methanol (MET) and trifluoroacetic acid (TFA). In addition, the influence of concentrations of TFA and ratio of MET:TFA on chromatographic separation and dete

Czech name

Určení thiolových sloučenin pomocí HPLC-ED

Czech description

Thiol compounds such as cystein (Cys), reduced (GSH) and oxidized (GSSG) glutathione and phytochelatins (PCs) play an important role in heavy metal detoxification in plants. These thiols are biological active compounds whose function is elimination of oxidative stress in plant cells. The aim of our work was to optimise sensitive and rapid method of high-performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of abovementioned thiol compounds in biological samples. An HPLC-ED system was consisted of two delivery pumps, chromatographic column Zorbax XDB (150.0 x 2.1 mm, 5 um particle size) and CoulArray detector containing eight working electrodes made from porous graphite. The most suitable isocratic mobile phase for the determination and separation of Cys, GSH, GSSG and PC2 consisted of methanol (MET) and trifluoroacetic acid (TFA). In addition, the influence of concentrations of TFA and ratio of MET:TFA on chromatographic separation and dete

Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Vitamins 2004 Targeted Nutritional Therapy

ISBN

80-7194-644-3

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

179-180

Publisher name

Univerzita Pardubice

Place of publication

Pardubice

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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