Simultaneous femtomole determination of cysteine, reduced andoxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high performance liquid chromatography with electrochemical detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00005198" target="_blank" >RIV/62156489:43210/05:00005198 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Simultaneous femtomole determination of cysteine, reduced andoxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high performance liquid chromatography with electrochemical detection

Original language description

Thiol compounds such as cysteine (Cys), reduced (GSH) and oxidized (GSSG) gluathione, and phytochelatins (PCs) play an important role in heavy metal detoxification in plants. These thiols are biological active compounds whose function is elimination of oxidative stress in plant cells. The aim of our work was to optimise sensitive and rapid method of high performance liquid chromatography coupled with electrochemical detector (HPLC ED) for determination of the abovementioned thiol compounds in maize (Zeamays L.) kernels. Newapproach for evaluation of HPLC ED parameters is described. The most suitable isocratic mobile phase for the separation and detection of Cys, GSH, GSSG and PC2 consisted of methanol (MeOH) and trifluoroacetic acid (TFA). In addition, the influence of concentrations of TFA and ratio of MeOH:TFA on chromatographic separation and detection of the thiol compounds were studied. The mobile phase consisting from methanol and 0,05% (v/v) TFA in ratio 97:3 (%; v/v) was found

Czech name

Současné femtomolové stanovení cysteinu, redukovaného a oxidovaného glutathionu a fytochelatinů v obilkách kukuřice (Zea mays L.)za použití vysokoúčinné kapalinové chromatografie s elektrochemickou detekcí

Czech description

Thiol compounds such as cysteine (Cys), reduced (GSH) and oxidized (GSSG) gluathione, and phytochelatins (PCs) play an important role in heavy metal detoxification in plants. These thiols are biological active compounds whose function is elimination of oxidative stress in plant cells. The aim of our work was to optimise sensitive and rapid method of high performance liquid chromatography coupled with electrochemical detector (HPLC ED) for determination of the abovementioned thiol compounds in maize (Zeamays L.) kernels. Newapproach for evaluation of HPLC ED parameters is described. The most suitable isocratic mobile phase for the separation and detection of Cys, GSH, GSSG and PC2 consisted of methanol (MeOH) and trifluoroacetic acid (TFA). In addition, the influence of concentrations of TFA and ratio of MeOH:TFA on chromatographic separation and detection of the thiol compounds were studied. The mobile phase consisting from methanol and 0,05% (v/v) TFA in ratio 97:3 (%; v/v) was found

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Volume of the periodical

1084

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

134-144

UT code for WoS article

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EID of the result in the Scopus database

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