Isolation of chromosomes from Picea abies and their analysis by flow cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F04%3A00002614" target="_blank" >RIV/62156489:43210/04:00002614 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation of chromosomes from Picea abies and their analysis by flow cytometry

Original language description

A high-yield method for preparation of suspensions of intact Norway spruce [Picea abies (L.) Karst.] chromosomes was developed for the first time. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatments with 0.625 mM hydroxyurea for 18 h and after 8 h recovery in distilled water with 0.05 % (m/v) colchicine for 8 h. These treatments resulted in 50 % metaphase indices. Synchronized root tips were fixed in 2 % formaldehyde for 10 min and chromosomes were released into a lysis buffer by mechanical homogenisation, producing 5 x 105 chromosomes from 50 root tips, at average. The isolated chromosomes were morphologically intact and suitable for flow cytometric analysis. Flow karyotypes obtained afterthe analysis of DAPI-stained chromosomes indicated a possibility to sort at least three different chromosome types.

Czech name

Izolace chromozomů z Picea abies a jejich analýza průtokovou cytometrií.

Czech description

Byla vyvinuta metoda izolace chromosomů z buněk smrku ztepilého (Picea abies). Jako zdroj sloužily kořenové špičky klíčních rosltin. Izolované chromozomy byly morfologicky intaktní a mohly tak být využity pro průtokovou analýzu. Ukázalo se, že pomocí níje možno izolovat nejmémě tři různé chromozomy smrku ztepilého

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

<a href="/en/project/GV521%2F96%2FK117" target="_blank" >GV521/96/K117: Novel approaches for effective analysis and mapping of genomes in cultivated plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

199-2003

UT code for WoS article

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EID of the result in the Scopus database

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