An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00000638" target="_blank" >RIV/62156489:43210/05:00000638 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/05:00021190

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

Original language description

The electroanalytical determination of avidin in solution, in a carbon paste, and in a transgenic maize extract was performed in acidic medium at a carbon paste electrode (CPE). The oxidative voltammetric signal resulting from the presence of tyrosine and tryptophan in avidin was observed using square-wave voltammetry. The process could be used to determine avidin concentrations up to 3 fM (100 amol in 3 mu l drop) in solution, 700 fM (174 fmol in 250 mu l solution) in an avidin-modified electrode, and174 nM in a maize seed extract. In the case of the avidin-modified CPE, several parameters were studied in order to optimize the measurements, such as electrode accumulation time, composition of the avidin-modified CPE, and the elution time of avidin. Inaddition, the avidin-modified electrode was used to detect biotin in solution (the detection limit was 7.6 pmol in a 6 mu l drop) and to detect biotin in a pharmaceutical drug after various solvent extraction procedures. Comparable studi

Czech name

Analýza avidinu a biotinu a jejich interakce na attomolové hladině pomocí voltametrických a chromatografických technik

Czech description

Analýza avidinu a biotinu a jejich interakce na attomolové hladině pomocí voltametrických a chromatografických technik.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

381

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

1167-1178

UT code for WoS article

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EID of the result in the Scopus database

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