Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00006911" target="_blank" >RIV/62156489:43210/05:00006911 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/05:06G00201

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation

Original language description

The aim of the study was to elucidate the effect of induced leukocytes migration into bovine mammary gland on the manifestations of early and late apoptotic features of neutrophils cultivated in vitro. The neutrophil early apoptotic features were detected by the cytometric detection (FCM) phosphatidyl-serine translocation and DNA fragmentation. Late neutrophil apoptotic features were detected by ELISA quantitation histon-complexed DNA fragments. Leukocyte influx induction was accomplished by using fourinducers: i) sterile buffered saline solution (PBS); ii) 5% glucose solution (GLU); iii) synthetic muramyl dipeptide analogue (MDP); and iv) lipopolysaccharide (LPS), administered into the mammary gland lumen. After three-hour incubation, light microscopy examination revealed the highest statistically significant (P<0.05) expression of neutrophil apoptosis after induction by GLU. The lowest, though statistically insignificant apoptosis manifestation was detected by MDP and LPS. Determina

Czech name

Vliv některých induktorů migrace leukocytů do mléčné žlázy skotu na apoptózu neutrofilů v následné in vitro kultivaci.

Czech description

Předmětem studie bylo stanovit vliv indukované migrace leukocytů do mléčné žlázy skotu na manifestaci časného a pozdního stádia apoptózy neutrofilů kultivovaných in vitro.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/GA524%2F01%2F1290" target="_blank" >GA524/01/1290: Effects of antimicrobial agens on induction of apoptosis and necrosis of polymorphonuclear leukocytes during resolution of bovine mastitis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

305-312

UT code for WoS article

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EID of the result in the Scopus database

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